KMD Bioscience Co., Ltd.
400-621-6806
2018-12-25 Hits(746)
Q1: What types of expression systems and protein services does KMD Bioscience offer?
A1: Currently we offer E.coli, mammalian, Yeast, and Baculovirus (insect) expression systems. We offer extensive production and purification services, including tag-removal, lyophilization, endotoxin removal, fermentation, N-terminal Sequencing and QC testing. Our gene synthesis services also allow us to synthesize and subclone your gene to get it ready for protein production.
A2: Baculovirus (insect) expression is ideal for producing mature, folded, and processed recombinant proteins. In contrast to E. coli, insect cells are able to incorporate post-translational modifications, they are also particularly well-suited for expressing secreted proteins.
Q3: When should I use the Mammalian expression system?
A3: Proteins produced in mammalian cells have the advantage of containing full post-translational modifications (e.g. proper folding, glycosylation, phosphorylation, etc) of the expressed protein. However, mammalian expression levels tend to be lower, so the cost is higher and achieving large amounts of protein (>10mg) may be difficult.
Q4: What do you need to get started? Can you use my construct?
A4: We have codon optimization and gene synthesis services, so if you just have your sequence as a FASTA or NCBI entry, we can use that to get started. Or if you already have your cDNA in an expression vector we can use your provided construct as well. We would also request that you complete our protein order form so that we can understand your requirements.
Q5: Do you use an affinity tag to purify my protein? What if I need the tag removed?
A5: Yes, we will use an affinity tag (e.g. HIS or GST) to purify your protein. For difficult to express proteins, we may even try alternate tags (including SUMO or thioredoxin) to aid in solubility and expression. If needed, we can definitely cleave the tag and provide tagless protein as the final product.
Q6: Are there limits on what proteins you can produce?
A6: Our experienced protein team will do what it takes to purify almost any protein. However, for best success we recommend keeping the target protein <100kDa and avoiding proteins with too many transmembrane domains. We also recommend removing any signal peptides that may be attached to the N-terminus of the protein, as these can inhibit protein expression in recombinant systems.
Q7: Can you produce bulk (milligrams to grams) amounts of protein?
A7: Yes, for E. coli expression systems we are capable of producing large amounts of protein in a single lot (up to gram amounts!). For these large scale projects, we will typically start with a small-scale pilot expression and purification, where we will purify ~5mg of protein and send it to you for testing. If it meets your needs we can then scale up the production.
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Email：info@kmdbioscience.com
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