KMD Bioscience Co., Ltd.
400-621-6806
2019-03-14 Hits(1267)
In general, proteomics research involves investigating any aspect of a protein such as structure, function, modifications, localization or protein interactions. To investigate how particular proteins regulate biology, researchers usually require a means of producing (manufacturing) functional proteins of interest. Recombinant protein is a manipulated form of protein, which is generated in various ways to produce large quantities of proteins, modify gene sequences and manufacture useful commercial products.
Choosing an appropriate protein expression system is the key to the success of recombinant protein expression. These expression systems are summarized in the table below and include bacterial, yeast, insect, mammalian. Each system has advantages and challenges.
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Most common application |
Advantages |
Challenges |
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*Structural analysis
*Antibody generation
*Functional assays
*Protein interactions |
*Scalable
*Low cost
*Simple culture conditions |
*Protein solubility
*May require protein-specific optimization
*May be difficult to express some mammalian proteins |
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*Structural analysis
*Antibody generation
*Functional assays
*Protein interactions
|
*Eukaryotic protein processing
*Scalable up to fermentation (grams per liter)
*Simple media requirements |
*Fermentation required for very high yields
*Growth conditions may require optimization |
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*Function assays
*Structural analysis
*Expression of intracellular proteins
*Expression of protein complexes
*Virus production |
*Similar to mammalian protein processing
*Can be used in static or suspension culture |
*More demanding culture conditions than prokaryotic systems
*Production of recombinant baculovirus vectors is time consuming |
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*Function assays
*Structural analysis
*Antibody production
*Expression of complex proteins
*Protein interactions
*Virus production |
*High-level protein processing
*Can produce proteins either transiently, or by stable expression
*Robust optimized transient systems for rapid, ultrahigh-yield protein production |
*Gram-per-liter yields only possible in suspension cultures
*More demanding culture conditions |
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