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Alpaca Polyclonal Antibody Production Services

KMD Bioscience has been working on producing polyclonal antibodies for many years. We can offer comprehensive polyclonal antibody production services, which involve peptide or protein antigen synthesis, animal immunization, antibody purification and QC control test. Polyclonal antibodies can also be raised against a customer supplied antigen. Our scientific staff has extensive polyclonal antibody development experience, with upgraded antibody technology, we can develop high-quality polyclonal antibodies, and guarantee positive binding to antigen in ELISA assay.

Alpaca antibodies have the ability to withstand elevated temperatures and a wide pH range, which makes them of great interest for basic research and therapeutic applications. KMD Bioscience provides custom alpaca polyclonal antibody production services to the scientific community. We can also provide antigen design, peptide synthesis, gene expression, antibody purification, identification and antibody labeling services with our well-established and powerful antibody engineering platform.

 

Alpaca Monoclonal Antibody Production:

 

Unlike human Igs-consists of two heavy chains and two light chains, llamas, as all members of the camelid family, produce antibodies with an original scaffold only made of heavy chains (IgG2 and IgG3), called heavy Chain Antibody(hcAb), thus promoting binding to structural motifs unreachable before in protein targets, such as viral and enzymatic proteins. The hcAb contains a single variable domain (VHH) and two constant domains (CH2 and CH3). According to the report, cloned and isolated VHHs possess unique properties that enable them to excel conventional therapeutic antibodies and their smaller antigen-binding fragments in cancer targeting and therapy. VHHs express low immunogenicity, are highly robust and easy to manufacture and have the ability to recognize hidden or uncommon epitopes. The advantages of hcAb is significant: maintain stability even in 60°C, high tolerance to extremes in pH and temperature, and well expressed in microorganisms such as bacteria and yeast.

 

Service Process:

 

 

Service Content:

 

 

Protein

Peptide

Antigen Preparation

3-5mg

Molecular Weight>20 kDa

Concentration>0.5mg/ml

Purity>90%

~10mg

>12 amino acid

Purity>85%

Peptide Conjugation

 

KLH, BSA, OVA, Hydralink

Immunization and Sera Collection

Immunization and boosts of 1 alpaca/camel (~120 days), and ELISA screening the polyclonal sera.

Antibody Purification

Protein-A/G purification, Ion exchange resin, Antigen affinity chromatography and density gradient centrifugation

Antibody Modification(Optional)

HRP, AP, biotinylation

FITC, FAM conjugation

GST, His tag

Magnetic beads, Quantum dots

 

Service Highlights:

 

--Rich experience in polyclonal antibody development

--High affinity and specificity

--High ELISA titer: Guaranteed high ELISA titer -107

--Large scale production capability(all size runs available)

--Strict quality assurance: follow cGMP management rules

--Ideal source of therapeutic antibody

--An affordable alternative to monoclonal antibody production

--One-stop downstream services, including antigen production, antibody development, ELISA kit development, etc