Antibody Purification Service

As a professional provider of antibody and recombinant protein products and services, KMD Bioscience relies on years of pre-clinical research experiences and production experiences to provide upstream and downstream services for antigen and antibody production.


Antibody Purification - Protein A / G affinity Purification:


Protein A is a cell wall protein isolated from S. aureus binds to mammalian IgG via the Fc region. Protein A can be used to isolate and purify IgG and its subclasses and fragments. The Protein A agarose column is suitable for immunoprecipitation of murine IgG2a, IgG2b, IgA, human IgG1, IgG2 and IgG4.

Protein G is a cell wall protein isolated from G-type Streptococcus binds to mammalian IgG via the Fc region. Protein G Agarose is more suitable for immunoprecipitation of murine IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit and sheep polyclonal antibodies, and human IgG1 compared to Protein A Agrose, IgG2, IgG3 and IgG4.


Main Process:


Fig1 Protein A / G affinity Purification


Protein A/G Affinity Purification Packages:


*Customer provides antiserum, we will purify antibodies (IgG, IgM, IgD, IgA and IgE) according to customers’ specific requirements

*Purification scale: μg ~g

*Quality Report: antibody concentration, SDS-PAGE analysis results


Antibody Purification - Ligand Affinity Purification:


KMD Bioscience can provide ligand chromatography column according to the antibody or antigen supplied by the customer; as shown in Figure 2, beads are agarose gel column or magnetic beads (customized or recommended by us); linker can be added to reduce the steric effect between biomacromolecules.


Fig 2 ligand chromatography column


Applications of Affinity Chromatography:


*Purified biomacromolecules

*Concentration of dilute solution

*Storage of unstable proteins

*Remove residual contaminants from purified molecules

*Adsorption and purification of biomacromolecules without complementary ligands by immunosorbent

*Isolation of nucleic acids is an essential aspect of affinity chromatography applications


How to order?



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Hotline(1): +86-022-8216-4980          Hotline(2): +86-156-0119-0182


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