Antibody Purification Service

As a professional provider of antibody and recombinant protein products and services, KMD Bioscience relies on years of pre-clinical research experiences and production experiences to provide upstream and downstream services for antigen and antibody production.

 

Antibody Purification - Protein A / G affinity Purification:

 

Protein A is a cell wall protein isolated from S. aureus binds to mammalian IgG via the Fc region. Protein A can be used to isolate and purify IgG and its subclasses and fragments. The Protein A agarose column is suitable for immunoprecipitation of murine IgG2a, IgG2b, IgA, human IgG1, IgG2 and IgG4.

Protein G is a cell wall protein isolated from G-type Streptococcus binds to mammalian IgG via the Fc region. Protein G Agarose is more suitable for immunoprecipitation of murine IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit and sheep polyclonal antibodies, and human IgG1 compared to Protein A Agrose, IgG2, IgG3 and IgG4.

 

Main Process:

 

Fig1 Protein A / G affinity Purification

 

Protein A/G Affinity Purification Packages:

 

*Customer provides antiserum, we will purify antibodies (IgG, IgM, IgD, IgA and IgE) according to customers’ specific requirements

*Purification scale: μg ~g

*Quality Report: antibody concentration, SDS-PAGE analysis results

 

Antibody Purification - Ligand Affinity Purification:

 

KMD Bioscience can provide ligand chromatography column according to the antibody or antigen supplied by the customer; as shown in Figure 2, beads are agarose gel column or magnetic beads (customized or recommended by us); linker can be added to reduce the steric effect between biomacromolecules.

 

Fig 2 ligand chromatography column

 

Applications of Affinity Chromatography:

 

*Purified biomacromolecules

*Concentration of dilute solution

*Storage of unstable proteins

*Remove residual contaminants from purified molecules

*Adsorption and purification of biomacromolecules without complementary ligands by immunosorbent

*Isolation of nucleic acids is an essential aspect of affinity chromatography applications

 

How to order?

 

 

What can we help you with? Please contact us by:

Email(1): info@kmdbioscience.com   Email(2): kmdbioscience@gmail.com 

Hotline(1): +86-022-8216-4980          Hotline(2): +86-156-0119-0182

 

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