Cell Immortalization Service

Immortalized cell lines are cells that have been manipulated to proliferate indefinitely and can thus be cultured for long periods of time. They are either tumorous cells that do not stop dividing or cells that have been artificially manipulated to proliferate indefinitely and can, thus, be cultured over several generations to meet researcher needs. KMD Bioscience has been dedicated to the services of cell immortalization for many years. We have already successfully constructed the immortalized cell lines derived from human, mouse and rat. Based on the dedicated research teams and perfect experimental platforms, KMD Bioscience has created an efficient virus transfection technology. We offer a comprehensive range of reagents and engineered cell lines to meet your research needs. If you have any questions about production, please contact us. Our scientists will provide a reliable and risk-free immortalized cell line service to save your precious time and energy. We will escort your scientific research work!

 

Necessity of Cell Immortalization:

 

Although the primary cells have similar chromosome numbers as parent tissues and have specialized biochemical properties (growth factor and hormone secretion) as parent tissues, they have a limited lifespan/limited number of cell divisions, considerable variations in population and preparation, and are more susceptible to contamination. Cell immortalization could overcome those problems. Compared to primary cells, they have a tendency to grow more quickly and can grow up to higher cell density, a uniform cell type, and most donor sources are same therefore less batch-to-batch variation, they also maintain most cellular characteristics from parent cells. Using immortalized cell lines always saves money and time for long term projects.

 

 

Fig 1. Cell immortalization procedure

 

Cell Immortalization Methods:

 

Several methods of cell immortalization in mammal are as follows.

-- Simian virus 40 (SV40) cell immortalization system

-- The human telomerase reverse transcriptase (hTERT) cell immortalization system

-- Human papillomavirus (HPV) E6/E7 cell immortalization system

-- Epstein-Barr virus (EBV) cell immortalization system

-- MycT58A cell immortalization system

-- RasV12 cell immortalization system

 

Service Content:

 

-- Cell preparation

-- Primary cells for virus transduction

-- Immortalized cell line construction

-- qRT-PCR screening immortalized cells

-- Cell line quality control (reproduce for 12 to 15 generations)

-- Cell proliferation, frozen storage and delivery

 

Customer Provides:

 

-- The Target Cells

-- At least 4 tubes (>1 X 106 cells/tube and dry ice transport)

-- Culture conditions

If your cells require media other than DMEM and RPMI, we will require 1-2L of media and any applicable growth factors that you provide for the project.

 

Deliverables:

 

-- 2 tubes (>1 X 106 cells/tube) immortalized cells

-- Experimental report: morphological assessment; q-PCR analysis; Microbial contamination test results

 

Service Highlights:

 

-- Custom service: provide different immortalization methods according to customer needs

-- PCR analysis of transgene expression in detail

-- Short time and high efficiency

-- Affordable price with the best quality

 

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806.