Antibody Platform

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Service Line:+86-022-8216-4980

Address:Room 502, Building B15, Wuqing Developmental Area, Tianjin, China

Email:info@kmdbioscience.com

Goat Polyclonal Antibody Production Service

KMD Bioscience has been working on producing polyclonal antibodies for many years. With extensive experience in antibody production, we can offer comprehensive polyclonal antibody production services to the scientific community. Our capabilities enable us to provide complete support for alpaca, rabbit, goat, sheep and fully humanized antibody. Meanwhile, we can accept various antigens for polyclonal antibody production such as small molecules, peptides, modified peptides, proteins, fusion proteins, modified proteins, etc.

KMD Bioscience has experienced immunology experts, standard operating procedures, and standard animal facility, which can provide high-performance goat polyclonal antibody production services and related testing services. Moreover, we can ensure the ELISA titer is no less than 100,000 and all polyclonals we produced guarantee positive western blot result. At the same time, we also provide antigen customization services, including DNA synthesis, plasmid construction, protein expression, protein purification, protein modification, peptide synthesis, and small molecule structure analysis, etc.

 

Service Process:

 

 

Service Content:

 

Procedure

Description

Timeline

Immunization

1. Clients provide the antigens (small molecules, peptides, proteins); Purity>85%, concentration>0.5mg/mL, 3-5mg

2 Immunize one goat, 4-5 boosts, ELISA titre determination

3. Negative control: pre-immune serum collection, 1mL

4. Serum harvest and ELISA titre determination

5. Deliver ELISA report and 1 mL pre-immune serum

6-8 weeks

Consecutive bleed

1. Inject 500ug antigen per goat

2. 200-300mL blood is collected after every two weeks

3. Ship the product & reports to investigator to test the serum and determine the quality of the antibodies.

2-3 weeks/cycle

Total bleed

1. Collect all the blood and the goat or sheep is humanely euthanized.

2. Serum collection, provide the products according to investigator’s requirements

1-2 weeks

Purification

1. Purify the goat/sheep antisera with Protein A (antigen affinity purification is also available)

2. Deliver the purified polyclonal antibody to investigators

1 week

 

Besides, we can provide the following antibody services:

*Antibody Identification: ELISA, WB, IF, IP, IHC, SPR, etc.

*Antibody Modification: Enzyme modification (HRP, AP, biotinylation), fluorescent labeling (FITC, FAM, etc.), fusion label (GST, His), magnetic beads labeling, quantum dot labeling, etc.

 

Deliverable:

 

Materials

Description

Serum

1mL pre-immune serum and 250-500mL antisera

Antibody

DEAE purified antibody: 20-40mg

(10-20mg purified antibody will be delivered if investigator choose antigen affinity purification)

Report

Immunogen sequence, SDS-PAGE result, expression & clone sequencing report, ELISA titre result

According to investigator’s requirements, we can also provide the validation result of WB, IHC, IP, IF

 

Service Highlights:

 

--Rich experience in polyclonal antibody development

--High affinity and specificity

--Large scale production capability

--Strict quality assurance: cGMP management rules

--Guaranteed positive western blot result and high ELISA titer

--A variety of antibody purification techniques are available

--One-stop services, including antigen production, antibody development, antibody purification, titer determination and related services