Human Monoclonal Antibody Production Service

KMD Bioscience has been working on producing monoclonal antibodies for many years. Monoclonal antibodies are identical immunoglobulins that generated from identical immune cells. Compared to polyclonal antibodies, monoclonal antibodies can be specific to a single epitope and produced large quantities of identical antibodies. We can provide comprehensive services for high-quality monoclonal antibody development to meet various customers’ requirements. Our scientific staff has extensive monoclonal antibody development experience, deliver successful monoclonal antibodies to customers in every project.

There are two methods to produce human monoclonal antibody: Recombinant monoclonal antibody produced from phage display technology; Hybridoma cell line production by using cell fusion technology. With our upgraded antibody platform, KMD Bioscience provides one-stop services including antigen design, synthesis and modification, in vitro sensitization, phage library construction and screening, antibody modification and application verification.

 

Human Monoclonal Antibody Production:

 

After human body is infected by foreign substances such as viruses, bacteria and parasites, there are lymphocytes in the peripheral blood capable of secreting antibodies against these foreign substances. With our extensive antibody production experiences, we developed a unique platform to manufacture human monoclonal antibody. It is well known that EBV virus can infect and transform human cells, and the transformed human cells have the characteristics of the proliferation of tumor cells in vitro. At the same time, using peripheral blood mononuclear cells to perform in vitro sensitization, our engineers used isolated and sensitized peripheral blood mononuclear cells infected by EBV virus, forming a unique sensitized and screened monoclonal cells strategy. This strategy can improve the success rate of antigen specificity and high-affinity human monoclonal antibody production.

Human monoclonal antibody production via our hybridoma-like technology: clients can provide small molecules, peptides, modified peptides, proteins, fusion proteins, modified proteins as antigens for monoclonal antibody production. Meanwhile, we provide antigen custom services as well, such as DNA synthesis, plasmid construction, peptide synthesis, peptide modification, peptide and carrier protein cross-linking, small molecule structure analysis and carrier proteins conjugation.

 

Service Process:

 

 

Service Content:

 

Procedure

Description

Timeline

Peripheral blood mononuclear cell isolation and in vitro sensitization

1. Collecting peripheral blood from special populations and purifying monocytes

2. Culture monocytes in vitro and perform antigen sensitization

2-3 weeks

Hybridoma Production

1. EBV virus recovery and expansion

2. Monocyte immortalization: infection of cell cultures with EBV virus

3. Screening and cloning of hybridoma-like cells

4. After immunogen detection, select 1-10 positive clones, subcloning and expanded to 96-well plates

5. Screening positive subclones by ELISA and expansion of cultivation 

6. 1mL supernatant or positive clones delivered to investigator for testing

4-6 weeks

Functional Verification (Optional)

ELISA, WB, IF, FLAC, IHC, etc.

1-3 weeks

 

 

 

Service Highlights:

--Rich expertise in monoclonal antibody development

--High affinity and specificity—pM grade

--Antibodies produced from different sources: patients, rehabilitation and vaccine recipients

--Antibody humanization, can be used in CAR-T

--One-stop downstream services, including antigen production, antibody development, ELISA kit development, etc

 

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info.us@kmdbioscience.com or Hotline(1): +86-022-8216-4980; Hotline(2): +86-156-0119-0182

 

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