Insect Cell Protein Expression Service

  

 

 

 

KMD Bioscience has been working on recombinant protein expression and preparation for many years. Our scientists have extensive experience in insect cell protein expression, fermentation and purification and are able to quickly develop customised insect protein cell expression strategies for different customers. Insect baculovirus expression vector system has been a powerful protein expression system for intracellular protein production. It has a large genome, which enables the insertion of large exogenous genes, therefore, has the great advantage of expressing proteins with large molecular weight. It can also achieve complete post-translational modification and efficiently express exogenous genes. Insect cells can grow to high cell density and achieve high protein yield after being infected with baculovirus containing the target gene of interest.

KMD Bioscience has extensive experience in producing large quantities of recombinant proteins with the insect expression system. We can provide our customer with advanced codon optimization specific to Sf9, Sf21, Hi-5, and S2 insect cell lines, to further enhance the expression of protein. In the past couple of years, we have successfully produced about 1000 recombinant proteins using our own insect expression systems, including kinase proteins, viral proteins, cytokines and growth factor proteins.

 

Insect Host Cell Lines:

 

Cells

 

Source

Applications

Sf9

* Sf9 is separated from IPLBSF21-AE.

* Transfection, purification, production of high titer viruses, plating and expression of recombinant proteins. Sf21 cells are superior to Sf9 cells in some structural expression.

Sf21

* Sf21 is derived from IPLBSF-21 cell line

* Transfection, purification, production of high titer viruses, plating and expression of recombinant proteins. Sf21 cells are superior to Sf9 cells in some structural expression, such as crystal protein.

Hi5

* Hi5 is derived from the ovarian cells of the cabbage inchworm and cabbage looper

* Suitable for the expression of recombinant virus particles and also commonly used for transfection and plaque purification, and Hi5 cell line is more suitable for the expression of secreted recombinant proteins.

S2

* S2 is derived from the late embryonic cell of drosophila. Most of them are female tetraploid cells and some are diploid cells.

* More suitable for recombinant expression of viral structural proteins.

 

Service Procedure:

 

Steps

Specification

Timeline

Deliverables

Subcloning

* Gene synthesis and codon optimization
* Subcloning into expression vector

2 weeks

* Construct report

Culture expansion of virus

* Titer test(108-109) and verification of protein expression
* P3 virus production (Optional)

3-4 weeks

* 5-10 ml, >107 pfu/ml recombinant baculovirus
* Experiment report

Screening of yeast expression strains

* Detection of recombinant protein expression

2 weeks

* Positive clone strains
* Screening report

Expression optimization and testing

* P3 virus transfected insect cells
* SDS-PAGE &WB

1-2 weeks

* Images
* Experiment report

Large-scale protein expression and purification

* The recombinant proteins were purified by affinity chromatography, ion exchange chromatography and gel filtration (1L, 5L,10L, 30L, 80L,130L, 250L),≥90% purity
* SDS-PAGE

1-2 weeks

* 1~5mg recombinant protein
* Protein purification report
* Tag removal and separation of tag-free protein (if requested)

 

Service Process:

 

 

Service Highlights:

 

--Codon optimization: including rare codon analysis, protein hydrophilicity analysis and prediction of transmembrane domains

--High efficiency and short cycle

--A variety of fusion tag vectors and hosts to ensure high soluble protein expression and activity

--High-throughput and large-scale protein expression and production

--Various scale-up capacities ranging from 10L, 80L, 130L, 250L to 500L

--Low endotoxin level: LAL <0.1EU/ug

--One-stop services, including protein purification, protein sequencing, protein labeling and related services

 

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806;