Insect Cell Protein Expression Services

  

 

 

 

KMD Bioscience has been striking on recombinant protein expression and antibody development for many years, and we understand the need for impurities, to produce clean, reproducible results. Insect baculovirus expression vector system has been a powerful protein expression system for intracellular protein production. It has a large genome, which enables the insertion of large exogenous genes, therefore, has the great advantage of expressing proteins with large molecular weight. It also can to achieve complete post-translational modification and efficiently express exogenous genes. Insect cells can grow to high cell density and achieve high protein yield after being infected with baculovirus containing the target gene of interest.

KMD Bioscience has extensive experiences in producing large quantities of recombinant proteins with the insect expression system. We can provide our customer with advanced codon optimization specific to Sf9, Sf21, Hi-5, and S2 insect cell lines, to further enhance the expression of protein. In the past couple of years, we have successfully produced about 1000 recombinant proteins using our own insect expression systems, including kinase proteins, viral proteins, cytokines and growth factor proteins.

 

Insect Host Cell Lines:

 

Cells

Source

Applications

Sf9

Sf9 cells are derived from the IPLBSF-21 cell line.

Transfection, purification, production of high titer viruses, plating and expression of recombinant proteins. Sf21 cells are superior to Sf9 cells in some structural expression.

Sf21

Sf21 is a late embryonic cell of drosophila. Most of them are female tetraploid cells and some are diploid cells.

More suitable for recombinant expression of viral structural proteins.

Hi5

Hi5 is a late embryonic cell of drosophila. Most of them are female tetraploid cells and some are diploid cells.

More suitable for recombinant expression of viral structural proteins.

S2

S2 is a late embryonic cell of drosophila. Most of them are female tetraploid cells and some are diploid cells.

More suitable for recombinant expression of viral structural proteins.

 

Service Procedure:

 

Steps

Specification

Timeline

Deliverables

Subcloning

* Gene synthesis and codon optimization
* Subcloning into expression vector

2 weeks

* Construct report

Culture expansion of virus

* Titer test108-109) and verification of protein expression
* P3 virus production (Optional)

3-4 weeks

* 5-10 ml, >107 pfu/ml recombinant baculovirus
* Experiment report

Screening of yeast expression strains

* Detection of recombinant protein expression

2 weeks

* Positive clone strains
* Screening report

Expression optimization and testing

* P3 virus transfected insect cells
* SDS-PAGE &WB

1-2 weeks

* Images
* Experiment report

Large-scale protein expression and purification

* The recombinant proteins were purified by affinity chromatography, ion exchange chromatography and gel filtration (1L, 5L,10L, 30L, 80L,130L, 250L)≥90% purity
* SDS-PAGE

1-2 weeks

* 1~5mg recombinant protein
* Protein purification report
* Tag removal and separation of tag-free protein (if requested)

 

Service Process:

 

 

Service Highlights:

 

--Codon optimization: including rare codon analysis, protein hydrophilicity analysis and prediction of transmembrane domains

--High efficiency and short cycle

--A variety of fusion tag vectors and hosts to ensure high soluble protein expression and activity

--High-throughput and large-scale protein expression and production

--Various scale-up capacities ranging from 10 L, 80L, 130L, 250L to 500 L

--Low endotoxin level: LAL <0.1EU/mg

--One-stop services, including protein purification, protein sequencing, protein labeling and related services

 

How to Order?

 

 

What can we help you with? Please contact us by:

Email(1): info@kmdbioscience.com   Email(2): kmdbioscience@gmail.com 

Hotline(1): +86-022-8216-4980         Hotline(2): +86-156-0119-0182

 

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