KMD Bioscience Co., Ltd.
Service Line：+86-022-8216-4980
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
KMD Bioscience has been striking on recombinant protein expression and antibody development for many years, and we understand the need for impurities, to produce clean, reproducible results. Insect baculovirus expression vector system has been a powerful protein expression system for intracellular protein production. It has a large genome, which enables the insertion of large exogenous genes, therefore, has the great advantage of expressing proteins with large molecular weight. It also can to achieve complete post-translational modification and efficiently express exogenous genes. Insect cells can grow to high cell density and achieve high protein yield after being infected with baculovirus containing the target gene of interest.
KMD Bioscience has extensive experiences in producing large quantities of recombinant proteins with the insect expression system. We can provide our customer with advanced codon optimization specific to Sf9, Sf21, Hi-5, and S2 insect cell lines, to further enhance the expression of protein. In the past couple of years, we have successfully produced about 1000 recombinant proteins using our own insect expression systems, including kinase proteins, viral proteins, cytokines and growth factor proteins.
Insect Host Cell Lines:
|
Cells |
|
|
Source |
Applications |
|
Sf9 |
Sf9 cells are derived from the IPLBSF-21 cell line. |
Transfection, purification, production of high titer viruses, plating and expression of recombinant proteins. Sf21 cells are superior to Sf9 cells in some structural expression. |
|
Sf21 |
Sf21 is a late embryonic cell of drosophila. Most of them are female tetraploid cells and some are diploid cells. |
More suitable for recombinant expression of viral structural proteins. |
|
Hi5 |
Hi5 is a late embryonic cell of drosophila. Most of them are female tetraploid cells and some are diploid cells. |
More suitable for recombinant expression of viral structural proteins. |
|
S2 |
S2 is a late embryonic cell of drosophila. Most of them are female tetraploid cells and some are diploid cells. |
More suitable for recombinant expression of viral structural proteins. |
Service Procedure:
|
Steps |
Specification |
Timeline |
Deliverables |
|
Subcloning |
* Gene synthesis and codon optimization |
2 weeks |
* Construct report |
|
Culture expansion of virus |
* Titer test（108-109) and verification of protein expression |
3-4 weeks |
* 5-10 ml, >107 pfu/ml recombinant baculovirus |
|
Screening of yeast expression strains |
* Detection of recombinant protein expression |
2 weeks |
* Positive clone strains |
|
Expression optimization and testing |
* P3 virus transfected insect cells |
1-2 weeks |
* Images |
|
Large-scale protein expression and purification |
* The recombinant proteins were purified by affinity chromatography, ion exchange chromatography and gel filtration (1L, 5L,10L, 30L, 80L,130L, 250L)，≥90% purity |
1-2 weeks |
* 1~5mg recombinant protein |
Service Process:
Service Highlights:
--Codon optimization: including rare codon analysis, protein hydrophilicity analysis and prediction of transmembrane domains
--High efficiency and short cycle
--A variety of fusion tag vectors and hosts to ensure high soluble protein expression and activity
--High-throughput and large-scale protein expression and production
--Various scale-up capacities ranging from 10 L, 80L, 130L, 250L to 500 L
--Low endotoxin level: LAL <0.1EU/mg
--One-stop services, including protein purification, protein sequencing, protein labeling and related services
How to Order?
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What can we help you with? Please contact us by:
Email(1): info@kmdbioscience.com Email(2): kmdbioscience@gmail.com
Hotline(1): +86-022-8216-4980 Hotline(2): +86-156-0119-0182
Related Services
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +86-022-8216-4980
Technical Support： +86-156-0119-0182