Antibody Platform

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Email:info@kmdbioscience.com

Murine Polyclonal Antibody Production Service

KMD Bioscience has experienced immunology experts, standard operating procedures, and standard animal facility, which can provide first-class antibody products and technical services to scientists and researchers. KMD Bioscience can provide rabbit, mouse, rat polyclonal antibody customization services to worldwide scientists.

Polyclonal Antibody Production Services- Murine Polyclonal Antibody Production Services

KMD Bioscience can ensure that all polyclonals produced in house can generated from any hosts, any antigens (5-10mg, purity >85%) with a positive WB result. We can provide a full protocol from antigen design to animal immunization and ensure the ELISA titer is greater than 3 x 104.

 

Guaranteed Murine Polyclonal Antibody Packages:

pAb Packages

Contents

Timeline

ELISA Guaranteed pAb Production

Immunization of 2 mouse/rats/guinea pig, 
antisera collection and protein A purified
ELISA titer> 1:50,000

8-10 weeks

WB Guaranteed pAb Production

Immunization of mouse/rats/guinea pig, antisera collection and protein A purified

WB testing by investigator

10-12 weeks

IHC Guaranteed pAb Production

Immunization of mouse/rats/guinea pig, antisera collection and protein A purified

IHC testing by investigator

10-12 weeks

Guaranteed phospho-specific pAb Production

Immunization of 5 mouse/rats/guinea pig, antisera collection and protein A purified, phosphorylated titer/non phosphorylated titer>8:1

Testing by investigator

12-14 weeks

Guaranteed DNA methylation pAb Production

Immunization of 5 mouse/rats/guinea pig, antisera collection and protein A purified,

Testing by investigator

12-14 weeks

Other guaranteed pAb Production

Besides the above services, we can also provide guaranteed  Acetylation, IP and CHIP pAb production services

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Murine Polyclonal Antibody Protocol Packages:

 

Protein

Peptide

Phospho-peptide

Peptide Synthesis

3-5mg

purity>90%

M.W.> 10 kDa

~10mg

>10 amino acids

Purity>90%

~10mg

>10 amino acids

Purity>90%

Peptide Conjugation

 

KLH, BSA, OVA, Hydralink

 

Immunization and sera collection

Yes

Yes

Yes

Titration by

ELISA, WB

(Optional: IF, IP, IHC)

ELISA, WB

(Optional: IF, IP, IHC)

ELISA, WB

(Optional: IF, IP, IHC)

Protein-A purification

Yes

Yes

 

Antigen-affinity purification

Optional

Optional

Yes

Antibody Modification (Optional)

Enzyme modification: HRP, AP, biotinylation

Labeling Services: FITC, FAM, Magnetic beads, Quantum Dot

Fusion tags: GST, His

 

Deliverable:

 

Contents

Purified Antibody

5~15 mg ProteinG/A purified antibody 

Report

Immunogen Sequence, SDS-PAGE, ELISA titer result

WB, IHC, IP, IF result (Optional)

 

Service Highlights:

*High affinity and specificity

*High ELISA titer: Guaranteed pAb ELISA titer ~106

* Large scale production capability (all size runs available)

*Strict quality assurance: cGMP management rules

*Several immunization protocols are available