Antibody Platform

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Service Line:400-621-6806

Address:FL-4, Building A5, International Enterprise Community, Tianjin, China

Email:info@kmdbioscience.com

Murine Polyclonal Antibody Production Service

KMD Bioscience has built up a mature antibody production platform with our well-established antibody engineering technology. With the extensive expertise in antibody production technology, we are pleased to offer comprehensive polyclonal antibody production services for our worldwide customers. KMD Bioscience has experienced immunology experts, standard operating procedures, and standard animal facilities, providing first-class antibody products and technical services to researchers.

KMD Bioscience provides custom polyclonal antibody production services with multiple host species options, such as mouse, rat, rabbit, and alpaca. All polyclonal antibodies we produced guarantee positive western blot result and high specificity. We can provide a full protocol from antigen design to animal immunization and ensure the ELISA titer is greater than 3x104.

 

Guaranteed Murine Polyclonal Antibody Production Services:

 

Project Name

Contents

Timeline

ELISA guaranteed pAb production

* Immunization of 2 mice/rats/guinea pigs,

antisera collection and protein A purified

* ELISA titer> 1:50,000

8-10 weeks

WB guaranteed pAb production

* Immunization of mouse/rats/guinea pig, antisera collection and protein A purified

* WB testing by the investigator

10-12 weeks

IHC guaranteed pAb production

* Immunization of mouse/rats/guinea pig, antisera collection and protein A purified

* IHC testing by the investigator

10-12 weeks

Phospho-specific guaranteed pAb production

* Immunization of 5 mice/rats/guinea pigs, antisera collection and protein A purified, phosphorylated titer/non-phosphorylated titer>8:1, Testing by the investigator

12-14 weeks

DNA methylation guaranteed pAb production

* Immunization of 5 mice/rats/guinea pigs, antisera collection and protein A purified

* Testing by investigator

12-14 weeks

Other guaranteed pAb production

* Besides the above services, we can also provide Acetylation, IP and CHIP guaranteed pAb production services

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Service Process:

 

 

 

Service Content:

 

 

Protein

Peptide

Phospho-peptide

Peptide synthesis

3-5mg

Purity>90%

M.W.> 10 kDa

~10mg

>10 amino acids

Purity>90%

~10mg

>10 amino acids

Purity>90%

Peptide conjugation

 

KLH, BSA, OVA, Hydra link

 

Immunization and Sera Collection

Yes

Yes

Yes

Titration by

ELISA, WB

(Optional: IF, IP, IHC)

ELISA, WB

(Optional: IF, IP, IHC)

ELISA, WB

(Optional: IF, IP, IHC)

Protein-A purification

Yes

Yes

 

Antigen-affinity purification

Optional

Optional

Yes

Antibody modification (Optional)

* Enzyme modification: HRP, AP, Biotinylation

* Labeling Services: FITC, FAM, Magnetic beads, Quantum dot

* Fusion tags: GST, His

 

Deliverable:

 

 

Contents

Purified Antibody

* 5~15 mg Protein G/A purified antibody 

Report

* Immunogen sequence, SDS-PAGE, ELISA titer result

* WB, IHC, IP, IF result (Optional)

 

Service Highlights:

 

--Rich experience in polyclonal antibody development

--High affinity and specificity

--High ELISA titer: Guaranteed ELISA titer 106

--Large scale production capability

--Strict quality assurance: cGMP management rules

--Several immunization protocols are available

--One-stop services, including antigen production, antibody development, antibody purification, titer determination and related services

 

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806;