Antibody Platform

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Murine Polyclonal Antibody Production Service

KMD Bioscience has been working on producing polyclonal antibodies for many years. With the extensive expertise in the field of antibody production technology, we are pleased to offer comprehensive polyclonal antibody production services for our worldwide customers. KMD Bioscience has experienced immunology experts, standard operating procedures, and standard animal facility, which can provide first-class antibody products and technical services to researchers.

KMD Bioscience provides custom chicken polyclonal antibody production services with multiple host species options, such as mouse, rat, rabbit, and alpaca. All polyclonal antibodies we produced guarantee positive western blot result and high specificity. We can provide a full protocol from antigen design to animal immunization and ensure the ELISA titer is greater than 3x104.

 

Guaranteed Murine Polyclonal Antibody Production Services:

 

Project Name

Contents

Timeline

ELISA guaranteed pAb production

Immunization of 2 mice/rats/guinea pigs,

antisera collection and protein A purified

ELISA titer> 1:50,000

8-10 weeks

WB guaranteed pAb production

Immunization of mouse/rats/guinea pig, antisera collection and protein A purified

WB testing by investigator

10-12 weeks

IHC guaranteed pAb production

Immunization of mouse/rats/guinea pig, antisera collection and protein A purified

IHC testing by investigator

10-12 weeks

Guaranteed phospho-specific pAb production

Immunization of 5 mice/rats/guinea pigs, antisera collection and protein A purified, phosphorylated titer/non phosphorylated titer>8:1. Testing by investigator

12-14 weeks

Guaranteed DNA methylation pAb production

Immunization of 5 mice/rats/guinea pigs, antisera collection and protein A purified

Testing by investigator

12-14 weeks

Other guaranteed pAb production

Besides the above services, we can also provide guaranteed  Acetylation, IP and CHIP pAb production services

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Service Process:

 

 

Service Content:

 

 

Protein

Peptide

Phospho-peptide

Peptide synthesis

3-5mg

purity>90%

M.W.> 10 kDa

~10mg

>10 amino acids

Purity>90%

~10mg

>10 amino acids

Purity>90%

Peptide conjugation

 

KLH, BSA, OVA

 

Immunization and sera collection

Yes

Yes

Yes

Titration by

ELISA, WB

(Optional: IF, IP, IHC)

ELISA, WB

(Optional: IF, IP, IHC)

ELISA, WB

(Optional: IF, IP, IHC)

Protein-A purification

Yes

Yes

 

Antigen-affinity purification

Optional

Optional

Yes

Antibody modification (Optional)

Enzyme modification: HRP, AP, Biotinylation

Labeling Services: FITC, FAM, Magnetic beads, Quantum dot

Fusion tags: GST, His

 

Deliverable:

 

 

Contents

Purified Antibody

5~15 mg Protein G/A purified antibody

Report

Immunogen sequence, SDS-PAGE, ELISA titer result

WB, IHC, IP, IF result (Optional)

 

Service Highlights:

 

--Rich experience in polyclonal antibody development

--High affinity and specificity

--High ELISA titer: Guaranteed ELISA titer -106

--Large scale production capability

--Strict quality assurance: cGMP management rules

--Several immunization protocols are available

--One-stop services, including antigen production, antibody development, antibody purification, titer determination and related services