KMD Bioscience Co., Ltd.
Service Line：+86-022-8216-4980
Address：Room 502, Building B15, Wuqing Developmental Area, Tianjin, China
Email：info@kmdbioscience.com
 |
Polyclonal Antibody Production Services- Murine Polyclonal Antibody Production Services
KMD Bioscience can ensure that all polyclonals produced in house can generated from any hosts, any antigens (5-10mg, purity >85%) with a positive WB result. We can provide a full protocol from antigen design to animal immunization and ensure the ELISA titer is greater than 3 x 104.
Guaranteed Murine Polyclonal Antibody Packages:
|
pAb Packages |
Contents |
Timeline |
|
ELISA Guaranteed pAb Production |
Immunization of 2 mouse/rats/guinea pig, antisera collection and protein A purified ELISA titer> 1:50,000 |
8-10 weeks |
|
WB Guaranteed pAb Production |
Immunization of mouse/rats/guinea pig, antisera collection and protein A purified
WB testing by investigator |
10-12 weeks |
|
IHC Guaranteed pAb Production |
Immunization of mouse/rats/guinea pig, antisera collection and protein A purified
IHC testing by investigator |
10-12 weeks |
|
Guaranteed phospho-specific pAb Production |
Immunization of 5 mouse/rats/guinea pig, antisera collection and protein A purified, phosphorylated titer/non phosphorylated titer>8:1
Testing by investigator |
12-14 weeks |
|
Guaranteed DNA methylation pAb Production |
Immunization of 5 mouse/rats/guinea pig, antisera collection and protein A purified,
Testing by investigator |
12-14 weeks |
|
Other guaranteed pAb Production |
Besides the above services, we can also provide guaranteed Acetylation, IP and CHIP pAb production services |
-- |
Murine Polyclonal Antibody Protocol Packages:
|
|
Protein |
Peptide |
Phospho-peptide |
|
Peptide Synthesis |
3-5mg
purity>90%
M.W.> 10 kDa |
~10mg
>10 amino acids
Purity>90% |
~10mg
>10 amino acids
Purity>90% |
|
Peptide Conjugation |
|
KLH, BSA, OVA, Hydralink |
|
|
Immunization and sera collection |
Yes |
Yes |
Yes |
|
Titration by |
ELISA, WB
(Optional: IF, IP, IHC) |
ELISA, WB
(Optional: IF, IP, IHC) |
ELISA, WB
(Optional: IF, IP, IHC) |
|
Protein-A purification |
Yes |
Yes |
|
|
Antigen-affinity purification |
Optional |
Optional |
Yes |
|
Antibody Modification (Optional) |
Enzyme modification: HRP, AP, biotinylation
Labeling Services: FITC, FAM, Magnetic beads, Quantum Dot
Fusion tags: GST, His |
||
Deliverable:
|
|
Contents |
|
Purified Antibody |
5~15 mg ProteinG/A purified antibody |
|
Report |
Immunogen Sequence, SDS-PAGE, ELISA titer result
WB, IHC, IP, IF result (Optional) |
Service Highlights:
*High affinity and specificity
*High ELISA titer: Guaranteed pAb ELISA titer ~106
* Large scale production capability (all size runs available)
*Strict quality assurance: cGMP management rules
*Several immunization protocols are available
Room 502, Building B15, Wuqing Developmental Area, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +86-022-8216-4980
Technical Support： +86-156-0119-0182