Pinpoint Targeted Integration Cell Line Services

Integrating exogenous plasmid DNA into the chromosome of host cells, and then express the target protein for a long time, which is called stable cell line. High-quality stable cell line plays an important role in biological research, including drug development, gene function research and recombinant antibody production. However, constructing an effective cell line is a complex and time-consuming task. KMD Bioscience has rich experience in stable cell line construction, our cell lines can be directly applied to downstream biological research. We have successfully constructed a large number of target cell lines for many clients. Based on advanced technology and dedicated research teams, KMD Bioscience can provide the following services:

 

CRISPR/Cas9 Knock-out Cell Line Services

IOS Overexpression Stable Cell Line Services

IOS RNAi Stable Cell Line Services

Pinpoint Targeted Integration Cell Line Services

 

Pinpoint Targeted Integration Cell Line Construction:

 

When studying protein functional domains, it is necessary to mutate one or more bases accurately. Similarly, it is necessary to add a fluorescent protein or label to the N-terminus or C-terminus of the target protein when studying protein localization. KMD Bioscience can provide pinpoint targeted integration cell line services based on our advanced CRISPR/Cas9 system. The application of this platform is as follows:

 

--Gene marker: Adding a fluorescent protein or label to the N-terminus or C-terminus of the target protein.

--Pinpoint targeted mutation: Accurate mutation of one or more bases of the target gene.

--Pinpoint targeted integration: Any gene fragment can be integrated into any site of the genome.

 

Service Procedure:

 

 

Service Highlights:

 

--Customizable: We customize our offering to meet your specific project needs and provide experienced scientific support every step of the way.

--Extensive experience: Our scientists are experts at performing gene editing with CRISPR, from designing gRNA constructs for CRISPR to transfection and single clone generation of a wide range of cells.

--Pinpoint targeted integration rather than random integration.

--Stable inheritance, the gene will not be lost in the process of passage.

--Exogenous gene integration efficiency is not affected by fragment size.

 

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806;