KMD Bioscience Co., Ltd.
Service Line：+86-022-8216-4980
Address：Room 502, Building B15, Wuqing Developmental Area, Tianjin, China
Email：info@kmdbioscience.com
Integrating exogenous plasmid DNA into the chromosome of host cells, and then express the target protein for a long time, which is called stable cell line. High-quality stable cell line plays an important role in biological research, including drug development, gene function research and recombinant antibody production. However, constructing an effective cell line is a complex and time-consuming task. KMD Bioscience has rich experience in stable cell line construction, our cell lines can be directly applied to downstream biological research. We have successfully constructed a large number of target cell lines for many clients so far. Based on advanced technology and dedicated research teams, KMD Bioscience can provide the following services:
When studying protein functional domains, it is necessary to mutate one or more bases accurately. Similarly, it is necessary to add a fluorescent protein or label to the N-terminus or C-terminus of the target protein when studying protein localization. KMD Bioscience can provides pinpoint targeted integration cell line services based on our advanced CRISPR/Cas9 system. The application of this platform is as follows:
* Gene marker: Adding a fluorescent protein or label to the N-terminus or C-terminus of the target protein.
* Pinpoint targeted mutation: Accurate mutation of one or more bases of the target gene.
* Pinpoint targeted integration: Any gene fragment can be integrated into any site of the genome.
Service Procedure:
Service Highlights:
*Customizable: We customize our offering to meet your specific project needs and provide experienced scientific support every step of the way.
*Extensive experience: Our scientists are experts at performing gene editing with CRISPR, from designing gRNA constructs for CRISPR to transfection and single clone generation of a wide range of cells.
*Pinpoint targeted integration rather than random integration.
*Stable inheritance, the gene will not be lost in the process of passage.
*Exogenous gene integration efficiency is not affected by fragment size.
Room 502, Building B15, Wuqing Developmental Area, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +86-022-8216-4980
Technical Support： +86-156-0119-0182