Plasmid DNA Preparation Services

KMD Bioscience has been working on molecular biology research for many years. Plasmid vector is a powerful tool for genetic engineering. The quality of DNA obtained is critical factor for genetic experimentation. We can provide comprehensive plasmid DNA preparation services from microgram- to gram-scale, to meet research institutes and biotechnology industries customers’  requirements.

With our upgraded DNA preparation platform, KMD Bioscience offers several different levels of research grade and preclinical grade plasmid production. We have rich experience in plasmid extraction, all plasmids we manufactured guarantee strict quality control, to ensure that the highest quality product will be delivered to our customers. A variety of our packages are designed to support diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research, etc.

 

Service Type:

 

KMD Bioscience offers three different grades of plasmid production:

 

Options

Application

Research Grade

* Molecular cloning

* Mutagenesis

* Southern blotting

* Library construction

Transfection Grade

* Transfection and co-transfection

* Virus packaging

* Protein manufacturing

* Antibody production

* Gene, vaccine, and gene therapy studies

* Animal studies

Preclinical Grade

* Virus production

* DNA vaccines

* Preclinical animal studies

 

Service Process:

 

 

Quality Control:

 

 QC Items

Specification

Appearance  

Clear, no visible particles

A260/280

1.8≤OD260/OD280≤2.0

Plasmid concentration

provide dry powder

Residual RNA

Non-detectable by gel electrophoresis

Genomic DNA

Non-detectable by gel electrophoresis

Endotoxin Assay

Transfection grade <0.1 EU/μg;

Preclinical grade<0.01 EU/μg

Bioburden Testing(optional add-on)

No growth on agar plate after 48 hours

Restriction analysis(optional add-on)

Conforming to reference pattern

Homogeneity(optional add-on)

Preclinical grade: 90% supercoiled

 

Service Highlights:

 

--Rich experience in plasmid extraction

--Different type of quality grades, from research to preclinical

--Quality-assured: Strict quality management, complete manufacturing documentation

--Microgram-to-gram-scale quantity, to meet various customers’ needs

--One-stop downstream services, including gene synthesis, custom cloning, or mutagenesis