Recombinant Mouse Monoclonal Antibody Production Services

KMD Bioscience has been working on antibody production research for many years. We have skilled team of scientists and standard operating procedures, which can provide comprehensive recombinant monoclonal antibody production services. Mouse monoclonal antibody has many features such as high specificity, stable passage and extensive applications in basic research, medical diagnosis and tumor therapy. Monoclonal antibody can be produced via traditional hybridoma technology or phage display technology. With extensive experience in the field of phage display technology, our scientists are pleased to construct high quality immunized mouse antibody libraries and screen out high affinity monoclonal mouse antibodies.

KMD Bioscience provides high-quality monoclonal antibody production services range from antigen design, synthesis and modification, animal immunization, phage library construction and screening to antibody modification. Meanwhile, we can develop monoclonal antibodies from various host species, including rat, rabbit, camel and other species.

 

Recombinant Mouse Monoclonal Antibody Generation:

 

KMD Biosciences can provide two methods of production of mouse monoclonal antibodies: recombinant monoclonal antibody produced from phage display technology; hybridoma cell line production by using cell fusion technology.

In the phage technique for the development of recombinant mouse monoclonal antibody, we cloned the variable regions of the murine antibody (Vλ, Vk, and VH) into the phagemid system with the M13 phage pIII fusion protein, subsequently screened for high specificity and high-affinity rabbit-derived antibody variable regions (Vλ, Vk, and VH) were cloned into plasmid vectors carrying mouse antibody scaffold proteins and transiently expressed in our recombinant expression platform (up to ~5 g/L).

 

Service Process:

 

 

Service Content:

 

Procedure

Carrier Type

Deliverable

Lead Time

Hapten Modification

/

Modified small molecule derivatives

LC-MS result

1-2 weeks

Complete Antigen Production

*Bovine serum albumin (BSA)

*Ovalbumin (OVA)

*Keyhole limpet hemocyanin (KLH)

*Human Serum Albumin (HSA)

*Human gamma globulins

Complete antigen with SDS-PAGE, UV or MALDI-TOF identification results

1-2 weeks

Phage Antibody Library Production

*Antigen emulsification

*Immunization

*Collection of splenocytes (or peripheral blood lymphocytes), variable region gene isolation

*Phagemid construction, transfection expression, library construction

*Antibody screening, re-cloning, antibody expression

PCR results, immunoassay results (including ELISA titer and specificity test)

1-2 months

 

Service Highlights:

 

--Rich experience in recombinant monoclonal antibody development

--High affinity and specificity

--Mature antibody and recombinant protein platform

--Strict quality assurance: cGMP management rules

--Guaranteed positive western blot result

--A variety of antibody purification techniques are available

--One-stop services, including antigen preparation, antibody development, phage library construction, antibody modification and related services