Stable Cell Line Construction Services

KMD Bioscience can provide you with STR approved, more than 300 tumor cells and primary cells. Including lung cancer cells, small-cell lung cancer cells, cervical cancer cells, intestinal cancer cells, gastric cancer cells, kidney cancer cells, liver cancer cells, thyroid cancer cells, esophageal cancer cells, hypopharyngeal cancer cells, breast cancer cells, ovarian cancer cells, endometrial adenocarcinoma cells, prostate cancer cells, colon cancer cells, colorectal cancer cells, colon adenocarcinoma cells, bladder cancer cells, cholangiocarcinoma cells, pancreatic cancer cells, laryngeal cancer cells, osteosarcoma cells, melanoma cells, glioblastoma cells, neuroblastoma cells, etc. Each type of cell is strictly managed and validated, easily infected by lentivirus or adenovirus and facilitating genetic manipulation. We are able to provide personalized solutions designed to supply researchers with the widest choice of cells to meet your research requirements.

 

Lentiviral Infection System

The Lentiviral infection system is an HIV-based gene therapy vector system. It is capable of infecting dividing and non-dividing cells and effectively integrating exogenous genes into host chromosomes, so that exogenous genes can be expressed persistently and stably.

KMD Bioscience used the second-generation self-inactivating lentiviral infection vector system to develop a variety of gene manipulation vector systems. At the same time, we have established various purification processes to package lentiviruses with different purity requirements. Our scientists have extensive experience in lentiviral infection experiments and we can provide you with high-quality stable cell line construction services.

 

Service Types

KMD Bioscience can offers different types of lentiviral vectors to meet various needs of our clients, including gene knock-down (KD), gene overexpression (OE), gene overexpression (Cas9-SAM), gene knock-out (KO) and gene knock-in (KI). We also provide high-titer lentiviral particles (≥1E+8 IU/ml) packages with different purity, which can infect target cells and then screening stable cell lines to meet the needs of scientific or clinical experiments.

 

References

1. Cockrell AS, Kafri T. Gene delivery by lentivirus vectors. Mol Biotechnol. 2007 Jul; 36(3):184-204.

2. Sakuma T, Barry MA, Ikeda Y. Lentiviral vectors: basic to translational. Biochem J. 2012 May 1; 443(3):603-18.