Yeast Protein Expression Service

 

 

 

Expression of proteins in yeast is a common alternative to prokaryotic and higher eukaryotic expression. Yeast cells offer many of the advantages of producing proteins in microbes (growth speed, easy genetic manipulation, low-cost media) while providing some of the attributes of higher eukaryotic systems (post-translational modifications, reasonable spatial folding).

KMD Bioscience provides comprehensive and high-quality customized recombinant protein expression services with yeast expression system. Based on our mature protein expression system and advanced protein expression technology, we can offer industry-scale protein fermentation services to meet high-throughput and large-scale production requirements. We have various fermentation tanks, bioreactors and protein purification systems, which can provide recombinant proteins from milligram level to gram level. In addition, we are devoted to providing one-stop services from the cDNA (gene) cloning to protein purification and downstream modifications such as tag addition-removal, endotoxin testing and removal, conjugations. We can offer flexible service solution tailored to your needs every step of the way.

 

Service Process:

 

Steps

Specification

Timeline

Deliverables

Gene synthesis, Cloning, Plasmid Prep

* Gene synthesis and Codon optimization

* Subcloning into appropriate expression vector

* Plasmid Prep and DNA QC

1 week

* Recombinant expression plasmid
* DH5α strains
* Sequencing report

Transformation of yeast expression strains

* Transform plasmids into appropriate yeast expression strains
* Amplification and culture of the above 5-10 positive clones
* SDS-PAGE

2 weeks

* Positive clone strains
* Screening report

Screening of yeast expression strains

* Detection of recombinant protein expression

2 weeks

* Positive clone strains
* Screening report

Protein expression evaluation and optimization

* Scale up protein expression using optimized conditions

2 weeks

 

Small-scale protein expression and purification

* The recombinant proteins were purified by affinity chromatography, ion exchange chromatography and gel filtration (1L yeast solution)
* SDS-PAGE

1-2 weeks

* 1~10mg recombinant protein
* Protein purification report
* Tag removal and separation of tag-free protein (if requested)

Large-scale protein expression and purification

* 5L yeast solution(According to client requirements)

1-2 weeks

* According to client's requirements

Value-added services

* Detection of the quality of the target protein by HPLC, mass spectrometry(MS)

1-2 weeks

* According to client's requirements

 

Service Highlights:

 

--Codon optimization: including rare codon analysis, protein hydrophilicity analysis and prediction of transmembrane domains

--High efficiency and short cycle

--A variety of fusion tag vectors and hosts to ensure high soluble protein expression and activity

--Strict quality control

--Low endotoxin level: LAL <0.1EU/ug

--Customization: quantity, purity, tags, and endotoxin levels are customizable

--Multiple scale-up capacities, including small scale (500ml, 10L) and large scale (80L, 130L, 250L and 500L)

--One-stop services, including protein purification, protein sequencing, protein labeling and related services

 

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806;