Mouse Monoclonal Antibody Production Service

KMD Bioscience can provide two mouse monoclonal antibody production protocols to meet clients' different needs: 1. Cell fusion technique: including mouse hybridomas, mouse/rat, mouse/guinea pig cell fusion, and fully humanized hybridoma to produce monoclonal antibodies. 2. Phage display library construction technique. We can provide antigen design, peptide synthesis, gene expression, immunization, antibody purification, identification and antibody labeling services with our well-established and powerful Antibody Engineering Platform.

Monoclonal Antibody Production Services- Mouse Monoclonal Antibody Production Services

Production of mouse monoclonal antibodies by our hybridoma platform: Customers can provide small molecules, polypeptides, modified polypeptides, proteins, fusion proteins, modified proteins and the antigens for monoclonal antibody production. In the meanwhile, we also provide antigen customization services, including DNA synthesis, plasmid construction, protein expression, protein purification, protein modification, peptide synthesis, peptide modification, peptide and carrier protein cross-linking, small molecule structure analysis and carrier protein conjugation. Our lab staffs have many years of experience in the development of in vitro diagnostic antibody & antigens, we have unique experience in the selection and identification of antibodies (such as in vitro detection of antigen immunogenicity), please feel free to contact our customer service at any time, we will provide you the customized solutions and protocols.

Meanwhile, our patent adjuvant can cause strong immune response after 21 days of immunization. In addition, we also provide a faster way- surgical spleen cell immunization to save investigator’s time.

 

The mouse monoclonal antibody production service protocol is shown below:

Procedure

Description

Timeline

Immunization

1. Immunization of 5 mice (BALB/c), 3-4 times

2. Sera collection from 5 mice and ELISA titer determination

3. Immunization report will be delivered to investigator

4-6 weeks

Hybridoma Screening

1. Spleen cells (the highest serum titer) fused with mouse myeloma cells.

2.  Limiting dilution of fusion cells

3. HAT selection

4. Selection of 1-10 positive clones and subcloning to 96 plates

5. Screening positive subclones by ELISA and expansion of cultivation 

6. 1mL supernatant/positive clones delivered to investigator for testing

4-6 weeks

Production of Ascites Fluid (Optional)

1. The hybridoma cells selected by the investigator were used to produce ascites, mice were immunized, Protein A/G purified the ascites, and ELISA titer determination.

2.  Purified antibody is delivered to customer, <5mg/clone

3.  Provide detailed report and residual ascites antibody

1-3 weeks

Functional Verification (Optional)

ELISA, WB, IF, FLAC, IHC, etc.

1-3 weeks

 

Service Highlights:

 

*High affinity and specificity—pM grade

*Suitable for production of antibody with inactivated antigen

*Suitable for Immunohistochemistry of formaldehyde fixation

*Applicable to different levels of antibody screening protocols, such as epitope-specific antibody screening

*By using our FastAb Mouse Adjuvant, the antibody titer will peak after 3rd or 4th injection, decrease the immunization timeline and immunogen dosage.