KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
KMD Bioscience is a professional provider of antibody and recombinant protein products and services, with years of pre-clinical research experience and production experience to provide upstream and downstream services for antigen and antibody production. Whether you have a clearly defined protocol or you just have an idea for an experiment, KMD Bioscience can tailor it to your needs.
Antibody Purification - Protein A / G affinity Purification:
Protein A is a cell wall protein isolated from S. aureus that binds to mammalian IgG via the Fc region. Protein A can be used to isolate and purify IgG and its subclasses and fragments. The Protein A agarose column is suitable for immunoprecipitation of murine IgG2a, IgG2b, IgA, human IgG1, IgG2 and IgG4.
Protein G is a cell wall protein isolated from G-type Streptococcus that binds to mammalian IgG via the Fc region. Protein G Agarose is more suitable for immunoprecipitation of murine IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit and sheep polyclonal antibodies, and human IgG1 , IgG2, IgG3 and IgG4.
Main Process:
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Fig1 Protein A / G affinity Purification
Protein A/G Affinity Purification Packages:
--Customer provides antiserum, and we will purify antibodies (IgG, IgM, IgD, IgA and IgE) according to the customers' specific requirements
--Purification scale: μg ~g
--Quality Report: antibody concentration, SDS-PAGE analysis results
Antibody Purification - Ligand Affinity Purification:
KMD Bioscience can provide a ligand chromatography column according to the antibody or antigen supplied by the customer; as shown in Figure 2, beads are agarose gel column or magnetic beads (customized or recommended by us); linker can be added to reduce the steric effect between biomacromolecules.
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Fig 2 ligand chromatography column
Applications of Affinity Chromatography:
--Purified biomacromolecules
--Concentration of dilute solution
--Storage of unstable proteins
--Remove residual contaminants from purified molecules
--Adsorption and purification of biomacromolecules without complementary ligands by immunosorbent
--Isolation of nucleic acids is an essential aspect of affinity chromatography applications
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
