KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
The quality of service provided by KMD Bioscience is the result of many years of research. KMD scientists have extensive experience in insect cell protein expression, fermentation and purification and are able to quickly develop customized insect cell protein expression strategies for you based on your purposes. We are committed to reducing your time wastage by providing a better service in less time, because more time means more progress. The insect baculovirus expression vector system has been a powerful protein expression system for intracellular protein production. It has a large genome, which enables the insertion of large exogenous genes, therefore, has the great advantage of expressing proteins with large molecular weight. It can also achieve complete post-translational modification and efficiently express exogenous genes. Insect cells can grow to high cell density and achieve high protein yield after being infected with baculovirus containing the target gene of interest.
KMD Bioscience is a reliable partner to fuel the engine of your research. We have extensive experience in producing large quantities of recombinant proteins with the insect expression system. We can provide our customer with advanced codon optimization specific to Sf9, Sf21, Hi-5, and S2 insect cell lines, to further enhance the expression of the protein. Data speaks for itself, KMD has successfully produced about 1000 recombinant proteins using our own insect expression systems, including kinase proteins, viral proteins, cytokines and growth factor proteins.
Insect Host Cell Lines:
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Cells |
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Source |
Applications |
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Sf9 |
* Sf9 is separated from IPLBSF21-AE. |
* Transfection, purification, production of high titer viruses, plating and expression of recombinant proteins. Sf21 cells are superior to Sf9 cells in some structural expressions. |
|
Sf21 |
* Sf21 is derived from IPLBSF-21 cell line |
* Transfection, purification, production of high titer viruses, plating and expression of recombinant proteins. Sf21 cells are superior to Sf9 cells in some structural expressions, such as crystal protein. |
|
Hi5 |
* Hi5 is derived from the ovarian cells of the cabbage inchworm and cabbage looper |
* Suitable for the expression of recombinant virus particles and also commonly used for transfection and plaque purification, and Hi5 cell line is more suitable for the expression of secreted recombinant proteins. |
|
S2 |
* S2 is derived from the late embryonic cell of drosophila. Most of them are female tetraploid cells and some are diploid cells. |
* More suitable for recombinant expression of viral structural proteins. |
Service Procedure:
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Steps |
Specification |
Timeline |
Deliverables |
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Subcloning |
* Gene synthesis and codon optimization |
2 weeks |
* Construction report |
|
Culture expansion of virus |
* Titer test（108-109) and verification of protein expression |
3-4 weeks |
* 5-10 ml, >107 pfu/ml recombinant baculovirus |
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Screening of yeast expression strains |
* Detection of recombinant protein expression |
2 weeks |
* Positive clone strains |
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Expression optimization and testing |
* P3 virus transfected insect cells |
1-2 weeks |
* Images |
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Large-scale protein expression and purification |
* The recombinant proteins were purified by affinity chromatography, ion exchange chromatography and gel filtration (1L, 5L,10L, 30L, 80L,130L, 250L)，≥90% purity |
1-2 weeks |
* 1~5mg recombinant protein |
Service Process:
Service Highlights:
--Codon optimization: including rare codon analysis, protein hydrophilicity analysis and prediction of transmembrane domains
--High efficiency and short cycle
--A variety of fusion tag vectors and hosts to ensure high soluble protein expression and activity
--High-throughput and large-scale protein expression and production
--Various scale-up capacities ranging from 10L, 80L, 130L, 250L to 500L
--Low endotoxin level: LAL <0.1EU/ug
--One-stop services, including protein purification, protein sequencing, protein labeling and related services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-022-8216-4980;
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
