KMD Bioscience Ltd.

Knowledge Development and Management

KMD Bioscience Ltd. is located in Wuqing District, northwest of Tianjin, China. The R&D center is located in Hongshan District, Wuhan City, Hubei Province. KMD Bioscience is a comprehensive innovative technology application enterprise integrating R&D, production and sales. The company aims to provide scientists with high-quality experimental outsourcing services (CRO services), including experimental design, routine genetic engineering experiments, recombinant protein expression and purification, recombinant antibody production, natural protein isolation and purification, virus isolation and other immunology related services. We would like to introduce our research platforms:

 

--- Phage Antibody Display Technology Platform

KMD Bioscience utilizes its own phage display (M13, T7 phage display technology platform) to provide customers with cost-effective recombinant Vhh antibody (Nanobody/Camel Nano Antibody) customization protocols, including animal immunization, library establishment, library screening, and in vitro expression of recombinant antibodies.

 

---Antibody Engineering Technology Platform

KMD Bioscience's antibody engineering platform includes monoclonal antibody production service platform, polyclonal antibody production service platform, and antibody sequencing and transformation platform.

Monoclonal Antibody Production Platform: Based on the hybridoma technology-based mouse monoclonal antibody technology platform, it can obtain high-affinity and high-specificity monoclonal antibodies in 2-3 months. At the same time, based on phage display technology, we can produce recombinant monoclonal antibodies to different species, including mice, rabbits, camels, alpaca, and human monoclonal antibodies.

Polyclonal Antibody Production Service Platform can provide rabbit polyclonal antibody, sheep polyclonal antibody, camel polyclonal antibody, bovine polyclonal antibody and other multi-species antibody preparation services. According to different antigen types, the senior antibody experts from KMD Bioscience will design the experiments to meet every customer's requirements.

Antibody Sequencing Platform: In order to meet the needs of a large number of clients for antibody gene sequence and amino acid sequence information, we provide full-length amino acid sequence analysis and N-terminal sequencing services of De Novo sequencing service.

Antibody Modification Technology Platform: KMD Bioscience's antibody engineering platform includes chimeric antibody preparation, antibody affinity maturation, and antibody humanization services. At present, we have successfully produced more than a dozen chimeric antibodies and humanized antibodies, which have been successfully delivered to the customers.

 

---Protein Expression and Purification Service Technology Platform

KMD Bioscience currently has four protein expression platforms that provide customers with comprehensive and superior protein expression technology services. 

 

Mammalian Expression Platform: KMD Bioscience currently has cell-free CHO, Expi CHO, CHO-DG44, HEK293F, Vero and other cell lines for protein expression production. We use Gibco cell culture medium and related transfection reagents to ensure the high quality and high expression level of each batch of mammalian cell protein expression products. At the same time, KMD Bioscience has an expression vector system that is much higher than the commonly used protein expression vectors in the market, and can provide customers with high-purity protein.

 

Insect Cell Expression Platform: At present, the insect-expressing cells commonly used by KMD Bioscience are SF9, and the medium is Gibco in order to provide customers with high quality products. Using this protein expression platform, we have successfully prepared a variety of human transmembrane proteins, including a variety of GPCR family member proteins.

 

Prokaryotic Expression Platform: E. coli expression system has become very mature after years of development. However, an excellent expression strategy is essential if you want to obtain good soluble and high-yield protein expression in the E. coli expression system. KMD Bioscience's team of experts has massive experience and knowledge in protein expression and antibody production, and a very specific protocol will be provided to every customer.

 

Yeast Protein Expression Platform: Pichia pastoris, Saccharomyces cerevisiae and other protein expression systems, which are suitable for project requirements that require eukaryotic expression of protein, and it can make sure the yield and quality.

 

Plant Expression Platform: In order to provide better services, KMD Bioscience cooperates with domestic top research institutes to provide customers with plant protein expression services for transient transfection technology of genetic tobacco. The project requires only 4-6 weeks from design and construction to protein expression. The technology platform can provide customers with high-purity, high-quality plant-derived proteins in a short period of time, and has solved the problem that most plant proteins are insoluble or not expressed in the above-mentioned several protein expression systems.

 

--- Protein Fermentation Platform

Ultra-high-density fermentation technology: KMD Bioscience can provide 5L, 10L, 30L, 80L volume of high-density fermentation technology for adherent cells (microcarrier adherent culture technology), human cell adherent cells culture density up to 4x106 / ml, it's very suitable for high-yield expression fermentation of virus transfection protein, and results in 1g / L fermentation level. Using our platform, the recombinant antibody fermentation expression records up to 4.7 g/L fermentation level according to our records.

 

In addition, with the aerobic fermentation technology, we can provide ultra-high-density fermentation services of prokaryotic and yeast. The aerobic fermentation of prokaryotic fermentation technology makes the protein yield much higher than the traditional prokaryotic fermentation (the difference is close to 100 times).

  

--- Protein Purification Platform:

KMD Bioscience has Akta and BioRad Biological protein purifiers, and has full GE set of fillers, including Spedax 75/200, 500, G25, G100, CMFF, Q-FF, DEAE and pre-packed columns of different specifications. We can complete full set of protein purification service within 2 weeks. Our research team members are all from domestic biopharmaceutical companies. We have the ability to provide fast, high quality natural protein and transmembrane protein purification services.

 

--- Stable Cell Line Platform and Molecular Biology Platform

Stable Cell Line Construction Platform: KMD Bioscience can customize different types of stable cell line construction and screening services, ranging from multi-species, multi-cell line cell silencing, knockout, overexpression to other stable cells using Crisper/Cas9. At the same time, we could provide stable transfection cell line construction services based on electroporation or lipofection (Lipofectin2000/3000), such as CHO cell protein high expression cell lines.

 

Molecular Biology Platform of KMD Bioscience undertakes gene cloning, protein quantification, immunoblotting (SDS-PAGE, WB), host nucleic acid detection, antibody affinity purification, ELISA, endotoxin detection, HPLC analysis, Immunohistochemistry, IP, immune turbidity, flow cytometry and other related services.