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Antibody Humanization Service

KMD Bioscience has built up a mature antibody production platform with our well-established antibody engineering technology. Through the flourish of antibody-based therapeutic drugs, the demand of humanized antibody is synchronized growth. Immunogenicity can potentially lead to reduced efficacy through rapid clearance or neutralization of the drug or toxicity due to cross-reaction with proteins in the body, which can have catastrophic consequences for the patient. Antibody humanization is used to reduce the immunogenicity of animal monoclonal antibodies and for improve their activities in the human immune system. It is a very critical step in therapeutic antibody discovery process.

With our well-established antibody engineering platform, KMD Bioscience can offer efficient, and accurate antibody humanization service to the scientific community. We have developed antibody humanization strategies based on our phage display technology and we can preserve the specificity and affinity of the antibody toward the antigen whereas significantly or entirely eliminate the antibody's immunogenicity in humans. KMD Bioscience provides multiple strategies, such as human framework selection, CDR (Complementarity-determining regions) back mutation library screening, etc. Meanwhile, we are devoted to providing comprehensive technical services, including protein expression, antibody production, antibody purification, affinity analysis, and antibody affinity maturation.

 

Service Process:

 

 

Service Content:

 

KMD Bioscience can provide three antibody humanization strategies:

CDR-Grafting Antibodies: This process involves transferring the antibody CDRs into a proper human antibody framework, while retaining their original affinity and specificity. Such as grafting of the murine CDRs into human frameworks.

Chimeric Antibodies: The creation of chimeric antibodies (non-human variable regions with human constant regions) can partially alleviate immunogenic response. They were transfected to mammalian cells for recombinant antibody expression using DNA recombination technology.

Fully Humanized Antibodies: Using gene editing technology, the antibody gene in animal somatic cells were replaced by human, and the humanized antibodies was produced directly after animal immunization.

 

 

Service Highlights:

 

--Extensive experience in antibody engineering development

--Guaranteed high affinity and specificity

--High throughput expression analysis

--Detailed sequence analysis

--Strict quality assurance: cGMP management rules

--A variety of antibody humanization strategies are available

--One-stop services, including antibody development, antibody sequencing, antibody labeling and related services

 

How to Order?

 

 

What can we help you with? Please contact us by:

Email(1): info@kmdbioscience.com   Email(2): kmdbioscience@gmail.com 

Hotline(1): +86-022-8216-4980          Hotline(2): +86-156-0119-0182

 

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