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Stable Cell Line refers to the sustained and stable expression of a specific gene in a cell or interference with specific gene expression. The target gene plasmid DNA of the stably transfected cell strain is integrated into the chromosome of the cell, so that the cell stably expresses the gene for a long time. In the study of gene function, whether genes are efficiently transfected into target cells is one of the prerequisites for functional studies. The stable expression of the cell line compensates for the short-term defect of the foreign gene expression in some functional experiments of transient infection (or transfection), and facilitates long-term observation of the effect of the gene on cell function and protein-protein interaction.  

 

The constructed vector containing the target gene is introduced into the cell by transfection plasmid or virus infection, and the corresponding drug is selected according to the resistance markers contained in the different gene vectors to select and mix the positive clone. On the basis of the positive mixed clone, a positive monoclonal which grew from a single cell was obtained, and then a stably transfected cell line was obtained.

 

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