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Antibody Affinity Maturation Services

KMD Bioscience has been working on antibody research for many years. To achieve a certain level of therapeutic effect in vivo, the affinity of the antibody needs to reach a certain level. In addition, the affinity of an antibody decreased obviously after humanization. Therefore, it is necessary to increase the affinity of humanized antibodies to the pre-humanized level. Affinity maturation is a common strategy to improve antigen-binding affinity of antibodies. We offer antibody affinity maturation services that can improve the affinity of antibodies to a desired level.

Based on our well-established antibody phage display library, KMD Bioscience can provide affinity maturation services for multi-species antibodies. Our scientists have extensive experience in antibody affinity maturation, we are able to make the affinity of monoclonal antibodies reach 10-9M.

 

Antibody Affinity Maturation:

 

Affinity maturation is the process by which TFH cell-activated B cells produce antibodies with increased affinity for antigen during the course of an immune response. Successive generations of B cells mutate and present to the antigen, that recognize the antigen with high affinity will survive and the low affinity ones are eliminated. KMD Bioscience uses phage display library technology, combined with error-prone PCR and site-directed mutagenesis, we were able to mimic this over-frequency cellular mutations. Our technical advantage lies in the ability to mutate rapidly in vitro and construct more than 1010 independent clones of antibody phage display libraries, so that high specificity and affinity antibodies can be obtained in a short time.

 

Service Process:

 

 

Common Affinity Maturation Techniques:

 

In the process of antibody affinity maturation in vitro, the selection of mutation region is a key problem, site-directed mutagenesis or random mutagenesis can be performed on the CDR regions.

*Site-directed mutagenesis is based on 3D modeling in order to identify key residues involved in the antibody-antigen interaction. Specific mutations on these residues are performed in order to create a large phage display library with low junk variants and high probability of strong affinity enhancement.

*Random mutagenesis is inserted by the use of error prone PCR. This method allows the construction of large libraries containing 108 starting from 3 to 10 selected antibodies.

 

Deliverable:

 

Contents

Affinity optimized antibodies sequences

Purified antibody with high affinity

Comprehensive report and data: including SDS-PAGE and affinity analysis, etc

 

Service Highlights:

 

--Extensive experience and strong track record in antibody affinity maturation

--Improve the affinity up to 10-9M

--A variety of methods to ensure high success rate

--Strict quality control

--Various antibody library searching strategies are available

--One-stop services, including antibody development, antibody sequencing, antibody labeling, antibody humanization and related services