B Cell Immortalization Service

Since it was recognized that the Epstein-Barr Virus (EBV) could be used in vitro, to infect B cells and produce immortalized polyclonal B cell lines in culture, B cell immortalization has always been a research hotspot. B cells have a limited life in vitro culture and cannot proliferate indefinitely. However, the immortalization of B cells fundamentally solves the problem. B cell immortalization methods mainly include human B cell hybridoma technology, EBV transformation, lentiviral-mediated genetic modification, and in vitro induced culture of CD40L combined with multiple cytokines. Each method can be used alone or in parallel. Based on the leading-edge platform and profound knowledge in cell immortalization, the specialized and dedicated team at KMD Bioscience provides world-class solutions for B cell immortalization and helps you obtain the desired results. Our state-of-the-art technologies and highly qualified personnel guarantee that we can deliver maximum quality and flexibility in all phases of the service lifecycle.

 

EBV Infection:

 

Immortalization of B cells by EBV is an effective procedure for inducing longterm growth of certain B cells. The process can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells. Infection of primary B cells with EBV gives rise to growth-transformed and immortalized lymphoblastoid cell lines (LCL) in vitro. Among their many applications is the use of LCL to present antigens in a variety of immunologic assays and to generate human monoclonal antibodies.

EBV's hosts are highly targeted, which tend to infect only B cells in humans and certain primates, limiting its use in other species. KMD Bioscience has successfully immortalized B cells derived from humans, rats, mice and pigs by using this technology.

 

Fig 1. Immortalization of EBV-infected B cell

 

Applications of Immortalized B Cells:

 

The EBV-based immortalization technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As a result, EBV immortalized B cell lines are extensively used as a source of biological material for functional and molecular studies and represent a potentially limitless source of genomic DNA, expression RNA, and protein and system biology analysis. In addition, these cell lines can be used as a culture model for various studies, including functional and serological studies, molecular and genetic research, large-scale drug library screening, vaccine study, and high throughput biological studies.

 

Service Content:

 

-- Viral transduction of primary cells

-- Immortalized cell line construction services

-- Sorting of immortalized cells by flow cytometry

-- Cell line quality control (reproduce for 12 to 15 generations)

-- Cell proliferation, frozen storage and delivery

 

Customer Provides:

 

-- Isolated PBMC cells, at least 4 tubes, the number of cells in each tube > 106

 

Service Highlights:

 

-- Immortalized cell lines from any species and any tissues with the function you need

-- Reliable lab report with timely update

-- Characterization according to your requirements

-- Fast turnaround time

-- Affordable price with the best quality

 

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806.