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Phage Display Services

Phage display services utilize a phage-based display library to identify and develop particular protein and antibody interactions, is now one of the most potent and widely used laboratory technique for the study of protein and antibody drugs. KMD has been specialized in advanced phage display technologies for variety of service projects. With the mature antibody production platform we have built up, we can offer high-quality phage display library construction and custom phage display library screening services to meet various customer needs. KMD Bioscience now offers multi-species monoclonal antibody production services, such as human, rat, rabbit, chicken, sheep, goose, pig, bovine, horse, donkey, camel, alpaca, etc. With skilled technology, our scientists can offer high-quality phage display library construction and custom phage display library screening services to meet our clients’ different requirements.

Meanwhile, we are devoted to providing one-stop technical services including protein expression, affinity analysis, antibody humanization, antibody affinity maturation and related services. We are glad to use our extensive experience and advanced platform to offer the best service and the most qualified products to our customers.

 

Antibody Phage Display Library Platform:

 

Phage display technology (PDT) is a molecular diversity technology that allows the presentation of large peptide and protein libraries on the surface of filamentous phage. In 1985, Smith, G.P. proposed inserting exogenous genes into the modified phage shell protein and expressing fusion proteins containing exogenous proteins or polypeptides, called fusion phages. Through repeated adsorption-elution-amplification processes, phages that specifically bind to target proteins are screened from phage libraries. Then enrichment, amplification and gene sequencing were performed to infer the amino acid composition of exogenous proteins. A crucial advantage of this technology is the direct link between the experimental phenotype and its encapsulated genotype, which allows the evolution of the selected binders into optimized molecules. It has been successfully applied to epitope analysis, monoclonal antibody screening, determination of protein functional antagonist polypeptides or mimetic peptides and play an essential role in drug screening, vaccine design, etc.

 

Figure 1 Diagram of phage display technology

 

Technical process:

 

 In general, filamentous bacteriophages (e.g. M13) are the most popular vehicle choice for phage display and extensively used in many research field. Using M13 filamentous phage shell protein pIII and PVIII display technologies, KMD Bioscience can construct different kinds of natural or immune phage antibody display libraries for our clients, and the library capacity can reach 1011.

Specific polypeptides can be efficiently screened by the affinity between displayed polypeptides and target proteins (mainly refers to antibodies, antigens, some growth factor receptors, etc.) or other biological macromolecules. Firstly, the target protein was solidified, such as binding to a plastic plate (96-well plate), then adding phage libraries and reacting with it. After washing, the unbound phages were washed out and the affinity phages were captured. The captured phages were eluted and then infected with E. coli for further amplification. Such an "adsorption-elution-amplification" enrichment process is called panning. A round of panning can enrich 103 folds of the phages. Generally, short peptides interacting with target proteins can be screened from the phage libraries of 109-1010 after 3-4 rounds of panning.

 

 Figure 2 Workflow of phage display technology

 

Platform Highlights:

 

--Rapid production of soluble antigen: Combined with our recombinant protein expression system, we can product high-purity soluble antigen in a short time to support the immunization and screening of phage antibodies.

--Mature and stable immune library construction technology: We guarantee that the antibody affinity obtained by screening can even reach nM or pM grade.

--Fast and reliable candidate antibody screening and identification technology: According to different functional requirements of antibody drugs, a variety of screening systems have been developed, including direct screening, competitive screening, negative screening, cell screening, etc.

 

How to Order?

 

 

What can we help you with? Please contact us by:

Email(1): info@kmdbioscience.com   Email(2): kmdbioscience@gmail.com 

Hotline(1): +86-022-8216-4980         Hotline(2): +86-156-0119-0182

 

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Bovine Polyclonal Antibody Production Service Alpaca Polyclonal Antibody Production Services