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Phage Display Services

Based on our own phage display library platform, KMD Bioscience offers multi-species monoclonal antibody production services to meet different requirements, such as human, rat, rabbit, chicken, sheep, goose, pig, bovine, horse, donkey, camel, alpaca, etc. We have long-term devoted to the development and application of phage display technology. We are glad to use our extensive experience and advanced platform to offer the best service and the most qualified products to our clients.

 

Antibody Phage Display Library Platform

Phage display technology (PDT) is a molecular diversity technology that allows the presentation of large peptide and protein libraries on the surface of filamentous phage. In 1985, Smith, G.P. proposed inserting exogenous genes into the modified phage shell protein and expressing fusion proteins containing exogenous proteins or polypeptides, which called fusion phages. Through repeated adsorption-elution-amplification processes, phages that specifically bind to target proteins are screened from phage libraries. Then enrichment, amplification and gene sequencing were performed to infer the amino acid composition of exogenous proteins. A crucial advantage of this technology is the direct link that exists between the experimental phenotype and its encapsulated genotype, which allows the evolution of the selected binders into optimized molecules. It has been successfully applied to epitope analysis, monoclonal antibody screening, determination of protein functional antagonist polypeptides or mimetic peptides and play an important role in drug screening, vaccine design, etc.

 

Specific polypeptides can be efficiently screened by the affinity between displayed polypeptides and target proteins (mainly refers to antibodies, antigens, some growth factor receptors, etc.) or other biological macromolecules. Firstly, the target protein was solidified, such as binding to a plastic plate (96-well plate), then adding phage libraries and reacting with it. After washing, the unbound phages were washed out and the affinity phages were captured. The captured phages were eluted and then infected with E. coli for further amplification. Such an "adsorption-elution-amplification" enrichment process is called panning. A round of panning can enrich 103 folds of the phages. Generally, short peptides interacting with target proteins can be screened from the phage libraries of 109-1010 after 3-4 rounds of panning.

Platform Highlights:

Using M13 filamentous phage shell protein pIII and PVIII display technologies, KMD Bioscience can construct different kinds of natural or immune phage antibody display libraries for our clients, and the library capacity can reach 1011. Our platform features are as follows:

* Rapid production of soluble antigen: Combined with the company's recombinant protein expression system, we can product high-purity soluble antigen in a short time to support the immunization and screening of phage antibodies.

* Mature and stable immune library construction technology: We guarantee that the antibody affinity obtained by screening can even reach nM or pM grade.

* Fast and reliable candidate antibody screening and identification technology: According to different functional requirements of antibody drugs, a variety of screening systems have been developed, including direct screening, competitive screening, negative screening, cell screening, etc.