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Stem Cell Induction Differentiation Service

KMD Bioscience has been engaged in the field of cell research for many years. We have a team of professional research experts, a complete cell experiment platform, and an experienced and skilled experimental team, can provide customers with high-quality stem cell differentiation services.

IPSC (Induced pluripotent stem cells) are generated by artificially inducing non-pluripotent cells to express specific genes and possess the potential to differentiate into various types of somatic cells. At present, iPSC has the potential to be used in drug screening [1], disease treatment and research [2-3]. It's the same as growing stem cells, the method of differentiation depends on the type, species, target lineage, and somatic cell type of the stem cell. PCR techniques (such as real-time PCR), cell sorting/flow cytometry, immunocytochemistry, western blot and biomarker analysis can be used to analyze the lineage and identity of differentiated cell types. Considering the current lack of clinically relevant in vitro models for drug screening, development and research, it is of great significance to induce iPSC differentiation into target somatic cell models required for various studies.

 

 

Fig 1. Representative images of ventricular zone-like structures formed by newborn neurons (green) and neural progenitor cells (red) at week 4 of differentiation in brain-like organs [4]

 

 Mesenchymal stem cells (MSCS) are a kind of pluripotent stem cells with all the characteristics of stem cells, namely, the ability of self-renewal and pluripotent differentiation. MSC are widely distributed in vivo and have the potential of multidirectional differentiation. Under specific in vivo and in vitro environment, they can be induced to differentiate into a variety of tissue cells. In addition to various mature cells of mesoderm, MSC can also be transformed into nerve cells, liver cells, islet cells, epithelial cells and so on. MSC are abundant in source and easy to collect, especially suitable for cell therapy to promote tissue regeneration and treat genetic diseases, etc. Induction of differentiation of mesenchymal stem cells has very important research value.

KMD Bioscience is an experienced provider of iPSC and MSC differentiation services that can differentiate iPSC and MSC into various genealogy-targeted cell types to extend the scope of your research projects (e.g., drug discovery or screening, etc.). We are committed to providing high quality and comprehensive services for your scientific research, and we are happy to use our rich experience and advanced platform to provide the best service to meet every need of our customers.

 

Service Content:

 

Stem cell induction differentiation services available at KMD Bioscience:

 

Source

Cell type

Differentiated cell

IPSC

Neuronal Cells

* Neural stem cells (NSC), neural progenitor cells (NPC), mature astrocytes, dopaminergic neurons, motor neuron progenitor cells, cerebral cortex neurons, oligodendrocytes, dopamine neurons, etc.

Retinal pigment epithelium (RPE)

 

* High quality RPE with typical cobblestone morphology and pigmentation.

 

Myocardial Cells

 

* Ready-to-use and functional myocardial cells.

 

Immune Cells

* CD34+ progenitor cells, CD8+T cells, NK cells, monocytes and dendritic cells from cloned main iPSC lines.

Hepatocytes

* Ready-to-use and functional hepatocytes.

MSC

Mesoblastema cells

* Bone, fat, muscle, cartilage, and vascular endothelium.

Non mesodermal cells

* Nerve cells, liver cells, renal tubular epithelial cells, lung cells, pancreatic β cells.

 

KMD Bioscience can provide differentiation services including but not limited to the above types of cells, as well as various other types and species of stem cells to support our clients' research.

 

Deliverables:

 

-- One thin or two cell culture tubes that differentiate into specific cell types 

-- Experiment report (including test results)

 

Service Highlights:

 

-- Complete and advanced experimental instrument platform to meet stem cell induction and differentiation services

-- Mature stem cell differentiation technology system, high success rate

-- A variety of cell differentiation models to meet customer needs for various projects

--Rich experience in cell culture, customers do not need to worry about mycoplasma contamination

 

How to Order?

 

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806.

 

References:

[1] Sharma, R., George, A., Nimmagadda, M. et al. Epithelial phenotype restoring drugs suppress macular degeneration phenotypes in an iPSC model. Nat Commun 12, 7293 (2021). https://doi.org/10.1038/s41467-021-27488-x

[2] Kim, NS., Wen, Z., Liu, J. et al. Pharmacological rescue in patient iPSC and mouse models with a rare DISC1 mutation. Nat Commun 12, 1398 (2021). https://doi.org/10.1038/s41467-021-21713-3

[3] Burley, S., Beccano-Kelly, D.A., Talbot, K. et al. Hyperexcitability in young iPSC-derived C9ORF72 mutant motor neurons is associated with increased intracellular calcium release. Sci Rep 12, 7378 (2022). https://doi.org/10.1038/s41598-022-09751-3

[4] Zhao, J., Fu, Y., Yamazaki, Y. et al. APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer's disease patient iPSC-derived cerebral organoids. Nat Commun 11, 5540 (2020). https://doi.org/10.1038/s41467-020-19264-0